Plant Proteomic Research 3.0

Abstract

Advancement in high-throughput “Omics” techniques has revolutionized plant molecular biology research. Proteomics offer one of the best options for the functional analysis of the genome, generating detailed information that integrated with that obtained by other classic and -omics approaches will provided a deeper knowledge of the different plant processes Up to six different generations of proteomics platforms (gel, label, gel free/label free, targeted) have appeared in the last twenty years which are being exploited in describing protein profiles, PTMs and interactions. Nevertheless, the ultimate success of any proteomic strategy lies in the various factors including isolation of full component of proteins, separation, visualization and their accurate identification. Despite recent advancements, more emphasis needs to be given to the protein extraction protocols, especially for very low-abundant, hydrophobic, and large molecular weight. Thus, amalgamation of diverse MS techniques, complemented with genome-sequence data and modern bioinformatics analysis with improved sample preparation and fractionation strategies, offer a powerful tool to identify and characterize novel proteins and to follow temporal changes in protein relative abundances under different environmental conditions. Furthermore, post-translational modifications and protein-protein interactions provide deeper insight into protein molecular function. Authors are welcome to submit original research articles and reviews addressing recent advancements, as well as limitations of current proteomic techniques and their diverse applications to get new insights of plant molecular responses to various biotic and abiotic stressors and the molecular bases of other processes. Proteomics focus is also related to translational purposes, including food traceability and allergen detection. In addition, bioinformatic techniques are needed for a more confident identification, quantitation, data analysis and networking, especially with non-model, orphan, plants, including medicinal, and meditational plants as well as forest tree species. "Guest Editors: