Germination Of White Muscardine Fungus, Beauveria Bassiana (Bals.) Vuill. On Different Plant Extracts

Abstract

Laboratory experiment was conducted to evaluate the germination of white muscardine fungus Beauveria bassiana (Bals.) on different plant extract. B. bassiana was mass produced using unmilled rice as a substrate. Two-week-old culture of B. bassiana (GLH isolate) was used. Extracts from the leaves of test crops (rice, mungbean, peanut and soybean) was used and tested separately. Four serial dilutions (crude: 10,000 ppm; 1,000 ppm; 100 ppm) of the different extracts were made and tested separately. Germination of conidia was observed for 24 hours post-inoculation at room temperature. Germination was observed in the extracts of all plant species tested 24 hours postinoculation at room temperature. Highest germination rate of B. bassiana conidia was found in soybean (81.51%) followed by mungbean (51.91%), peanut (47.39%) and rice (36.94%). Germination of B. bassiana was also found to be significant among different levels of plant extracts. Highest germination was in 100 ppm (83.59%), 1,000 ppm (69.75%) extract concentration followed by 10,000 ppm (45.44%) and crude extract (18.97%). In general, germination rate was higher at lower extract concentration or germination rate was inversely proportional with level of plant extract. Conidia of B. bassiana germinated in different plant species extracts and at various concentrations. It is concluded, that there is a high probability that these plants extracts contain toxic substances/compounds with inhibitory effects on the germination of B. bassiana conidia.