Vui lòng dùng định danh này để trích dẫn hoặc liên kết đến tài liệu này: http://thuvienso.vanlanguni.edu.vn/handle/Vanlang_TV/15239
Toàn bộ biểu ghi siêu dữ liệu
Trường DCGiá trị Ngôn ngữ
dc.contributor.authorSpring, Allison M-
dc.contributor.authorDocherty, Kathryn M-
dc.contributor.authorDomingue, Kenneth D ...-
dc.date.accessioned2020-02-12T04:09:56Z-
dc.date.available2020-02-12T04:09:56Z-
dc.date.issued2018-
dc.identifier.issn1178-6221-
dc.identifier.urihttp://thuvienso.vanlanguni.edu.vn/handle/Vanlang_TV/15239-
dc.descriptionAir, Soil and Water Research Volume 11: 1–12 © The Author(s) 2018vi
dc.description.abstractDispersal of airborne microorganisms is an important ecological process, resulting in the distribution of bacteria to all habitats on Earth. Investigation of this process is limited by the ability to collect uncontaminated high-altitude microbial samples for use with next-generation sequencing approaches. Here, we describe the design of a Remote Airborne Microbial Passive sampling system. Troubleshooting experiments demonstrate that the samplers collect adequate DNA for bacterial 16S rRNA (ribosomal RNA) amplicon–based Mi-Seq sequencing at 2 and 150 m from the ground. When samplers are closed, they retain only a low number of sequences, and may be used as a negative control. We also demonstrate that the optimal amount of collection dishes to include in the sampler is 8, and that freezing collection dishes at −80°C is an alternative to immediate DNA extraction. Samplers may be used to address a variety of ecological and human health–related questionsvi
dc.language.isoenvi
dc.publisherSage Publications Ltd.vi
dc.subjectAtmospherevi
dc.subjectSamplervi
dc.subjectMicrobevi
dc.subjectBacteriavi
dc.subject16S rRNAvi
dc.subjectCommunityvi
dc.titleA Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communitiesvi
dc.typeOthervi
Bộ sưu tập: Bài báo_lưu trữ

Các tập tin trong tài liệu này:
Tập tin Mô tả Kích thước Định dạng  
BBKH754_TCCN_A Method for Collecting.pdf
  Giới hạn truy cập
A Method for Collecting Atmospheric Microbial Samples From Set Altitudes for Use With Next-Generation Sequencing Techniques to Characterize Communities1.83 MBAdobe PDFXem/Tải về  Yêu cầu tài liệu


Khi sử dụng các tài liệu trong Thư viện số phải tuân thủ Luật bản quyền.